Formation and Regulation Mechanism of Ascorbic Acid in Sweet Pepper and Chili Pepper at Different Growth Stages
The formation and regulation of ascorbic acid (vitamin C) in sweet pepper (Capsicum annuum L.) and chili pepper (Capsicum frutescens L.) are dynamic processes influenced by genetic, developmental, and environmental factors. During the early vegetative stage, biosynthesis is primarily regulated through the L-galactose pathway, with key enzymes such as GDP-mannose pyrophosphorylase and L-galactono-1,4-lactone dehydrogenase showing high activity. As the fruit transitions from immature green to ripening stages, the ascorbic acid content increases substantially, driven by enhanced synthesis and reduced degradation. Light intensity, temperature, and nutrient availability also modulate the expression of genes involved in ascorbic acid metabolism. Sweet peppers typically exhibit higher ascorbic acid accumulation than chili peppers, attributed to differences in metabolic flux and antioxidant enzyme regulation. Furthermore, the interplay between ascorbate peroxidase, glutathione reductase, and dehydroascorbate reductase ensures redox homeostasis throughout fruit development. Understanding these mechanisms provides insights into improving nutritional quality through genetic selection, optimized cultivation, and post-harvest handling strategies.
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